Diabetes Care, Vol 9, Issue 2 189-193, Copyright © 1986 by American Diabetes Association

ARTICLES

Evaluation of a new radioimmunoassay for urinary albumin

RG Brodows, D Nichols, G Shaker and NP Kubasik

We have evaluated a new commercially available radioimmunoassay kit for albumin determination in urine. The assay is precise; within-run precision (CV) in the clinically significant ranges is 1.8-3.5%, between-run, 1.2-8.5%. The minimum detection limit was 0.6 micrograms/ml. Analytic recovery of different concentrations of albumin added to urine ranged from 98% to 103%. Samples, stored in plastic containers, were stable at room temperature for periods up to 7 days. Mean albumin excretion rates, measured in 20 normal volunteers for 3-h and 24-h periods during the same day were similar (7.1 +/- 4.6 [SD] versus 6.5 +/- 5.0 micrograms/min). In 8 normal subjects, 3-h excretion rates measured daily for 5 days showed no significant variability. In eight insulin-dependent diabetic subjects, albumin excretion measured in short periods of urine collection (3 h) were also in close agreement with 24-h collections (24.7 +/- 28.9 versus 17.6 +/- 18 micrograms/min). From these results it appears that this commercially available kit is suitable for conveniently monitoring microalbuminuria in large numbers of patients in research studies as well as for office practice. Such widespread use should make it possible to better determine the clinical usefulness of this test in the management of diabetic patients.
CiteULike    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				T. Narita, M. Hosoba, M. Kakei, and S. Ito Increased Urinary Excretions of Immunoglobulin G, Ceruloplasmin, and Transferrin Predict Development of Microalbuminuria in Patients With Type 2 Diabetes Diabetes Care, January 1, 2006; 29(1): 142 - 144. [Full Text] [PDF]

				T. Narita, H. Sasaki, M. Hosoba, T. Miura, N. Yoshioka, T. Morii, T. Shimotomai, J. Koshimura, H. Fujita, M. Kakei, et al. Parallel Increase in Urinary Excretion Rates of Immunoglobulin G, Ceruloplasmin, Transferrin, and Orosomucoid in Normoalbuminuric Type 2 Diabetic Patients Diabetes Care, May 1, 2004; 27(5): 1176 - 1181. [Abstract] [Full Text] [PDF]

				G Berton, R Cordiano, R Palmieri, F Cucchini, R de Toni, and P Palatini Microalbuminuria during acute myocardial infarction; a strong predictor for 1-year mortality Eur. Heart J., August 2, 2001; 22(16): 1466 - 1475. [Abstract] [PDF]

				A. S. Reddi, V. R. Nimmagadda, A. Lefkowitz, H.-R. Kuo, and J. S. Bollineni Effect of Antihypertensive Therapy on Renal Injury in Type 2 Diabetic Rats With Hypertension Hypertension, August 1, 2000; 36(2): 233 - 238. [Abstract] [Full Text] [PDF]

				G. Berton, T. Citro, R. Palmieri, S. Petucco, S. Renzo De Toni, and P. Palatini Albumin Excretion Rate Increases During Acute Myocardial Infarction and Strongly Predicts Early Mortality Circulation, November 18, 1997; 96(10): 3338 - 3345. [Abstract] [Full Text]

				G. N. Jyothirmayi, I. Alluru, and A. S. Reddi Doxazosin Prevents Proteinuria and Glomerular Loss of Heparan Sulfate in Diabetic Rats Hypertension, May 1, 1996; 27(5): 1108 - 1114. [Abstract] [Full Text]