HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Extract Full Text (PDF) All Versions of this Article: dc07-1256v1 31/2/223    most recent Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Esmaillzadeh, A. Articles by Azadbakht, L. Search for Related Content PubMed PubMed Citation Articles by Esmaillzadeh, A. Articles by Azadbakht, L. Social Bookmarking                What's this?

Consumption of Hydrogenated Versus Nonhydrogenated Vegetable Oils and Risk of Insulin Resistance and the Metabolic Syndrome Among Iranian Adult Women

¹ Department of Nutrition, School of Public Health, Isfahan University of Medical Sciences, Isfahan, Iran
² Food Security and Nutrition Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Address correspondence and reprint requests to Ahmad Esmaillzadeh, PhD, Department of Nutrition, School of Public Health, Isfahan University of Medical Sciences, P.O. Box 81745, Isfahan, Iran. E-mail: esmaillzadeh{at}hlth.mui.ac.ir

Abbreviations: HVO, hydrogenated vegetable oil • SFA, saturated fatty acid • TFA, trans fatty acid

	INTRODUCTION

TOP INTRODUCTION RESEARCH DESIGN AND METHODS-- RESULTS-- CONCLUSIONS-- References

 
Remarkably rapid increase in the prevalence of the metabolic syndrome reflects the strong impact of lifestyle factors, including diet, on its etiology. In particular, dietary intake of fat may play a role in this regard. Different types of fat have been related to insulin resistance and the metabolic syndrome, but findings are inconsistent. One specific type of dietary fatty acid that has received increased attention is trans fatty acid (TFA); higher consumption was associated with increased risk of cardiovascular disease (1) and type 2 diabetes (2). Although some studies have reported a significant association between dietary fat intake and the metabolic syndrome (3,4), to our knowledge, such analyses have not been done separately for hydrogenated and nonhydrogenated vegetable oils. Hydrogenated vegetable oils (HVOs)—rich sources of both saturated fatty acids (SFAs) and TFAs—are extensively used for cooking in Iranian homes with average per-person intake of 14g per 1,000 kcal (5). In addition to large amounts of SFAs, these products have almost 33% of total fatty acids as TFAs. In other words, 4.2% of all calories consumed by Iranians are derived from TFAs, which is about twice the amount consumed in many developed countries (5). In the current study, we investigated whether high consumption of HVOs is contributing to a high prevalence of the metabolic syndrome among Iranian women.

	RESEARCH DESIGN AND METHODS—

TOP INTRODUCTION RESEARCH DESIGN AND METHODS-- RESULTS-- CONCLUSIONS-- References

 
This cross-sectional study was conducted among Tehrani female teachers aged 40–60 years selected by a multistage cluster random sampling method. Detailed information about this sample and measurements have been provided elsewhere (6). Altogether, 486 women, with informed written consent, participated in the current study. Dietary intakes were assessed using a valid 168-item semiquantitative food frequency questionnaire (7). HVO is commonly used for cooking in Iran, and margarine was considered an HVO. Therefore, HVOs here include both fully and partially hydrogenated vegetable oils. Sunflower, corn, canola, soybean, and olive oils were defined as nonhydrogenated vegetable oils (non-HVOs). Anthropometric indexes were measured as described previously (8). A blood sample was drawn after 12-h overnight fasting, and biochemical assessment was done (6). Blood pressure was measured three times, and the mean of the three measurements was considered the participant's blood pressure. Additional information regarding age, physical activity, smoking habits, menopausal status, socioeconomic status, medical history, and current use of medications was obtained using questionnaires. The metabolic syndrome was defined as recommended by the Adult Treatment Panel III (9). Insulin resistance was defined as the upper quartile of the homeostasis model assessment of insulin resistance score (10). To determine the associations, we used multivariable logistic regressions in different models.

	RESULTS—

TOP INTRODUCTION RESEARCH DESIGN AND METHODS-- RESULTS-- CONCLUSIONS-- References

 
Characteristics and dietary intakes of the study participants and multivariate-adjusted odds ratios (ORs) for the metabolic syndrome and insulin resistance are shown in Table 1. Those in the upper quintile of HVOs had higher age, BMI, and waist-to-hip ratio, were more likely to have insulin resistance, metabolic syndrome, and family history of diabetes, and had higher cholesterol intakes; whereas those in the upper quintile of non-HVOs had younger age, were slightly more physically active, less likely to have insulin resistance, and had lower intakes of energy as compared with those in the lowest quintiles. After control for age, energy intake, and other potential confounders, individuals in the highest quintile of HVOs were 2.48 (95% CI 1.51–4.92) and 2.71 (1.57–5.48) times more likely to have the metabolic syndrome and insulin resistance, respectively, compared with those in the lowest quintile, whereas those in the top quintile of non-HVOs had 44% (95% CI 3–74%) lower odds for insulin resistance compared with those in the lowest quintile. Further adjustment for dietary intakes had little impact on the associations. Additional control for BMI attenuated the associations, but they were still significant. No overall significant associations were seen between consumption of non-HVOs and the metabolic syndrome.

	CONCLUSIONS—

TOP INTRODUCTION RESEARCH DESIGN AND METHODS-- RESULTS-- CONCLUSIONS-- References

HVOs are used extensively in Iran. Commercial hydrogenation of vegetable oils results in production of TFAs, higher intakes of which have been documented to increase the risk of cardiovascular disease (11), diabetes (12), and elevated inflammatory biomarkers (13). Some investigators have reported no association between TFA intakes and insulin resistance (14,15). Others have shown ethnic differences in the relationship between dietary fat intake and insulin resistance (16). The Food and Drug Administration has ruled that TFA content of packaged foods marketed in the U.S. must appear on the Nutrition Facts panel, as of 1 January 2006. The TFA content of HVOs used in Iranian households is 23–36% (5). Given the importance of insulin resistance in the development of diabetes and heart disease, establishing appropriate levels of fat in the diet is an important clinical goal.

Although metabolic syndrome is highly prevalent in the world, limited data are available assessing the contribution of HVOs to such a high prevalence. However, numerous studies have reported the adverse effects of TFAs on serum lipid profiles (17). Vega-López et al. (18) have recently shown that partially hydrogenated soybean oils, compared with soybean and canola oils, adversely altered the lipoprotein profile in moderately hyperlipidemic subjects. In Iran, it has been estimated that between 11 and 39% of coronary heart disease events might be prevented by near-elimination of TFAs (5). Consistent with results of the present study, HVOs have been reported to be associated with insulin resistance; however, the clarity of this finding remains to be identified. Adverse effects of HVOs in this study could be attributed to both TFA and SFA content of these products. A favorable association of non-HVOs and insulin resistance could be explained by the beneficial effects of polyunsaturated fatty acid on glucose metabolism (19).

In conclusion, the present findings indicate that higher intakes of HVOs were associated with a greater risk of the metabolic syndrome and insulin resistance, whereas higher intakes of non-HVOs, in the range of energy requirements, were associated with a lower risk of insulin resistance.

	Acknowledgments

 
This manuscript was written based on data from a research project (contract number P. 25/47/2337) supported by the National Nutrition and Food Technology Research Institute of the Islamic Republic of Iran.

We thank the participants of the study for their enthusiastic support. None of the authors have any personal or financial conflicts of interest.

A.E. and L.A. participated in the collection of data, conception and design, statistical analysis and data interpretation, manuscript drafting, and approval of the final manuscript for submission.

	Footnotes

 
Published ahead of print at http://care.diabetesjournals.org on 13 November 2007. DOI: 10.2337/dc07-1256.

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C Section 1734 solely to indicate this fact.

Received for publication July 3, 2007. Accepted for publication October 30, 2007.

	References

TOP INTRODUCTION RESEARCH DESIGN AND METHODS-- RESULTS-- CONCLUSIONS-- References

 

1. Oomen CM, Ocke MC, Feskens EJ, van Erp-Baart MA, Kok FJ, Kromhout D: Association between trans fatty acid intake and 10-year risk of coronary heart disease in the Zutphen Elderly Study: a prospective population-based study. Lancet 357: 746–751, 2001[Medline]
   
2. Pisabarro RE, Sanguinetti C, Stoll M, Prendez D: High incidence of type 2 diabetes in peroxisome proliferator–activated receptor 2 Pro12Ala carriers exposed to a high chronic intake of trans fatty acids and saturated fatty acids. Diabetes Care 27: 2251–2252, 2004[Free Full Text]
   
3. Freire RD, Cardoso MA, Gimeno SG, Ferreira SR, Japanese-Brazilian Diabetes Study Group: Dietary fat is associated with metabolic syndrome in Japanese Brazilians. Diabetes Care 28: 1779–1785, 2005[Abstract/Free Full Text]
   
4. Grundy SM, Abate N, Chandalia M: Diet composition and the metabolic syndrome: what is the optimal fat intake? Am J Med 113: 25S–29S, 2002
   
5. Mozaffarian D, Abdollahi M, Campos H, Houshiarrad A, Willett WC: Consumption of trans fats and estimated effects on coronary heart disease in Iran. Eur J Clin Nutr 61: 1004–1010, 2007[Medline]
   
6. Esmaillzadeh A, Kimiagar M, Mehrabi Y, Azadbakht L, Hu FB, Willett WC: Fruit and vegetable intakes, C-reactive protein and the metabolic syndrome. Am J Clin Nutr 84: 1489–1497, 2006[Abstract/Free Full Text]
   
7. Azadbakht L, Mirmiran P, Esmaillzadeh A, Azizi F: Dairy consumption is inversely associated with the prevalence of the metabolic syndrome in Tehranian adults. Am J Clin Nutr 82: 523–530, 2005[Abstract/Free Full Text]
   
8. Esmaillzadeh A, Kimiagar M, Mehrabi Y, Azadbakht L, Hu FB, Willett WC: Dietary patterns, insulin resistance, and prevalence of the metabolic syndrome in women. Am J Clin Nutr 85: 910–918, 2007[Abstract/Free Full Text]
   
9. National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III): Third report of the national cholesterol Education Program (NCEP) Expert Panel on detection, evaluation, and treatment of high blood cholesterol in adults. Circulation 106: 3143–421, 2002[Free Full Text]
   
10. Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC: Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 28: 412–419, 1985[Medline]
    
11. Hu FB, Stampfer MJ, Manson JE, Rimm E, Colditz GA, Rosner BA, Hennekens CH, Willett WC: Dietary fat intake and the risk of coronary heart disease in women. N Engl J Med 337: 1491–1499, 1997[Abstract/Free Full Text]
    
12. Salmerón J, Hu FB, Manson JE, Stampfer MJ, Colditz GA, Rimm EB, Willett WC: Dietary fat intake and risk of type 2 diabetes in women. Am J Clin Nutr 73: 1019–1026, 2001[Abstract/Free Full Text]
    
13. Mozaffarian D, Pischon T, Hankinson SE, Rifai N, Joshipura K, Willett WC, Rimm EB: Dietary intake of trans fatty acids and systemic inflammation in women. Am J Clin Nutr 79: 606–612, 2004[Abstract/Free Full Text]
    
14. Lovejoy J, Champagne C, Smith S, et al: Relationship of dietary fat and serum cholesterol ester and phospholipid fatty acids to markers of insulin resistance in men and women with a range of glucose tolerance. Metabolism 50: 86–92, 2001[Medline]
    
15. Lovejoy JC, Smith SR, Champagne CM, Most MM, Lefevre M, DeLany JP, Denkins YM, Rood JC, Veldhuis J, Bray GA: Effects of diets enriched in saturated (palmitic), monounsaturated (oleic), or trans (elaidic) fatty acids on insulin sensitivity and substrate oxidation in healthy adults. Diabetes Care 25: 1283–1288, 2002[Abstract/Free Full Text]
    
16. Weigensberg MJ, Ball GD, Shaibi GQ, Cruz ML, Gower BA, Goran MI: Dietary fat intake and insulin resistance in black and white children. Obes Res 13: 1630–1637, 2005[Medline]
    
17. Lichtenstein AH, Ausman LM, Jalbert SM, Schaefer EJ: Effects of different forms of dietary hydrogenated fats on serum lipoprotein cholesterol levels. N Engl J Med 340: 1933–1940, 1999[Abstract/Free Full Text]
    
18. Vega-Lopez S, Ausman LM, Jalbert SM, Erkkila AT, Lichtenstein AH: Palm and partially hydrogenated soybean oils adversely alter lipoprotein profiles compared with soybean and canola oils in moderately hyperlipidemic subjects. Am J Clin Nutr 84: 54–62, 2006[Abstract/Free Full Text]
    
19. Clarke SD: Polyunsaturated fatty acid regulation of gene transcription: a mechanism to improve energy balance and insulin resistance. Br J Nutr 83: S59–S66, 2000[Medline]
    

CiteULike

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				A. Esmaillzadeh and L. Azadbakht Home use of vegetable oils, markers of systemic inflammation, and endothelial dysfunction among women Am. J. Clinical Nutrition, October 1, 2008; 88(4): 913 - 921. [Abstract] [Full Text] [PDF]

				A. Esmaillzadeh and L. Azadbakht Food Intake Patterns May Explain the High Prevalence of Cardiovascular Risk Factors among Iranian Women J. Nutr., August 1, 2008; 138(8): 1469 - 1475. [Abstract] [Full Text] [PDF]

This Article Extract Full Text (PDF) All Versions of this Article: dc07-1256v1 31/2/223    most recent Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Esmaillzadeh, A. Articles by Azadbakht, L. Search for Related Content PubMed PubMed Citation Articles by Esmaillzadeh, A. Articles by Azadbakht, L. Social Bookmarking                What's this?